9.4T Bruker SolariX XR 9.4 T FT-ICR equipped with a cryogenically cooled superconducting magnet. This instrument generates ions using an electrospray ionization (ESI) source, but it is commonly changed out for a nano-ESI source for analyzing very small samples which can only be generated on the nanogram and microgram scale. This instrument is capable of performing tandem mass spectrometry experiments using collision induced dissociation (CID), and is also equipped with an electron gun and a chemical ionization source which allow it to perform electron based dissociation techniques (ExD) like electron capture dissociation (ECD), electron detachment dissociation (EDD), electron transfer dissociation (ETD) and negative electron transfer dissociation (NETD). This instrument is frequently coupled to a capillary zone electrophoresis (CZE) with a CZE-MS interface in order to analyze complex mixtures of glycosaminoglycans, small molecules, or proteins. 12T Bruker SolariX XR 12 T FT-ICR equipped with a refrigerated superconducting magnet. This instrument is equipped with a CombiSource that has both electrospray ionization (ESI) and matrix assisted laser desorption (MALDI). Like the 9.4 T, this instrument has the ability to perform CID and ExD experiments which make this instrument useful for protein sequencing experiments. The high mass resolution of this instrument (up to 1,000,000 RP) allows it to distinguish between species with the same nominal mass. This high resolution allows it to achieve isotopic resolution on intact proteins and determine their molecular weight within 1 Da. The high mass accuracy of this instrument (<1 ppm) allows it to determine the elemental composition of molecules based on accurate mass alone. This instrument is available to users through the Proteomics and Mass Spectrometry Facility (PAMS). For inquiries about submitting samples for analysis, please refer to the PAMS website or contact drp@uga.edu. https://pams.uga.edu/ Types of analysis available: ESI or MALDI of small molecules: Elemental formula determination using accurate mass Elemental formula determination using isotopic fine structure Structural determination using CID or EID Complex sample analysis Laser desorption or ESI Analysis of atmospheric brown carbon Analysis of combustion products Protein analysis MALDI mass fingerprinting for protein identification Intact protein MS for molecular weight determination Native protein MS for the analysis of protein complexes Top-Down proteomics for PTM localization using CID, ECD, or ETD LCMS analysis available on request. Thermo Fisher UltiMate 3000 UHPLC+ is capable of standard LCMS and nano-flow LCMS experiments Thermo Fisher Orbitrap Elite with Agilent HP 3D Capillary Electrophoresis Instrument The Thermo Orbitrap Elite is also an FTMS instrument and represents one of the most widely used high resolution MS platforms. It has a maximum resolution of 240,000 (FWHM) without the need for a superconducting electromagnet. This instrument has a low resolution dual linear ion trap mass analyzer as well as a high resolution orbitrap mass analyzer. The orbitrap elite has a chemical ionization source that allows it to perform ETD experiments, but it has been modified to perform negative electron transfer dissociation (NETD). This instrument has an ESI source, a nanoESI source, and a CZE-MS interface. This instrument has been used to analyze synthetic and biologically derived mixtures of glycosaminoglycans. Waters Synapt G2 HDMS
